Bone defects often result from tumor resection, congenital malformation, trauma, fractures, surgery, or periodontitis in dentistry. Although dental implants serve as an effective treatment to recover mouth function from tooth defects, many patients do not have the adequate bone volume to build an implant. The gold standard for the reconstruction of large bone defects is the use of autogenous bone grafts. While autogenous bone graft is the most effective clinical method, surgical stress to the part of the bone being extracted and the quantity of extractable bone limit this method. Recently mesenchymal stem cell-based therapies have the potential to provide an effective treatment of osseous defects. In many clinical studies both the current therapy for bone regeneration and the perspectives in the field of stem cell-based regenerative medicine, addressing the sources of stem cells and growth factors used to induce bone regeneration effectively and reproducibly have been discussed.
In this regard, we have adapted a multidisciplinary approach towards engineering dental implants; through the systemic integration of multiple progenitor stem cells isolated from dental pulp tissues, growth factors to support cellular signaling and conductive extracellular matrix scaffold to be able to provide chemical as well as mechanical cues, thus promoting multicellular interactions, orchestrate cellular adhesion, migration and differentiation into a desired cellular lineage.
Accordingly, some indications that may denote a need for reconstructive, regenerative treatment of Dental Implant may include:
Difficulty in swallowing, chewing or biting of food.
Chronic jaw and/or Jaw joint pain and headache.
Chronic mouth breathing or dry mouth.
Immoderate wear to teeth.
Inequitable facial appearance.
Birth Defects or facial injuries.
Results of Dental Implants Through Dental Pulp Derived Stem Cells and Biodegradable Scaffolds.
They are essentially a substitute for natural root and are commonly in shape of a cylinder and a screw. Each implant is placed into a socket, carefully within the location of an intended tooth, with an aim to achieve immediate close association with surrounding bone, promoting functional restoration.
Both, dental pulp derived and neural crest derived stem cells can be efficiently utilized for regeneration of lost bony structure to achieve immediate close contact with the surrounding bone. This optimization of stability and regenerative power can be enhanced over the period of time to further support bone growth and its natural integration, to achieve desired outcome.
Stem Cell Treatment For Dental Pulp: Advancells Protocol
Chronic dental disease and tooth loss often lead to the loss of hard tissue in the jaw. Patients with missing teeth by infections, or inflammation may experience bone resorption with loss of the affected part of the jaw. In addition to making a patient uncomfortable, this bone loss can cause unsightly disfigurements and may complicate the fitting of implants and other dental appliances. Although dental implants serve as an effective treatment to recover mouth function from tooth defects, many patients do not have adequate bone volume to build an implant.
Thus, in order to adapt, circumvent and treat these local physiological as well as anatomical limitations; regenerative, reconstruction of bony platform with application of regenerative medicine and modern tissue engineering technology can be emphasized for natural, tailored lifting of sinus membrane, for their functional restoration.
In this regard, the site, size and associated soft tissue defects represent the three most important factors; in determining the impact of treatment procedure on functional restoration and aesthetics.
Our process of dental implants and reconstruction involves three key elements, which include:
Inductive soluble growth factors
Stem cells isolated from dental pulp tissue
Biocompatible scaffolds made up of Xeno/Allo/Synthetic substitutes
Accordingly, steps involved in the procedures are:
Step 1- Qualification For The Treatment
A safety and efficacy assessments will be prescribed by our experts in order to investigate a degree of severity and a need to undergo particular treatment. Following pretreatment assessments can be suggested.
Infectious Disease Testing
Medical Reports & History
Panorex X Rays
Cephalometric X Rays
Models & Impressions
As per the results, the patient will be further counselled for final treatment that will impact proper jaw functions and an aesthetic appearance of an entire face.
Step 2- Source Extraction
Mesenchymal stem cells and/or neural crest derived stem cells isolated from soft tissue of the wisdom tooth has been confirmed to be the most potent, non-invasive source of stem cells with high proliferation rate, higher degree of differentiation and a better capacity to interact with biocompatible matrices and growth factors for faster regeneration and healing.
Thus, Dental Pulp stem cells sourced from either wisdom tooth or milk tooth, that are already planned to remove will be collected as a better fitting tool due to easy access, and a very low morbidity of the anatomical site.
Step 3- Laboratory Processing
Tissue samples containing stem cells will be transported to our ultramodern GMP grade facility, through placing it, in a screw top vial containing appropriate transportation media; which may nourish it during transportation. Mesenchymal stem cells and/or neural crest derived stem cells will be isolated from a soft dental pulp tissue, which is encased inside a bony cavity of wisdom tooth or milk tooth; under controlled sterile conditions.
Step 4- Stem Cell Implantation
Expanded stem cells will be loaded on a suitable matrix called as scaffold to allow speedy attachment, faster regeneration into specific cell types and easy proliferation. Although scaffolds are of different types, shapes, patterns as well as biocompatible materials; depending upon the necessity, capacity and costing it can be made up of Xeno/Allo/Synthetic substitute.
Tissue Reconstruction through Biodegradable Scaffolds: - We have wide range of ideal scaffolds made up of different biocompatible materials, to provide chemical stability, physical strength and mimicking the surrounding tissue with respect to faster cellular proliferation, better adhesion performance, controlled degradation, easy differentiation and better integration.
A scaffold will further be implanted and secured in a position above and/or below jawline; depending upon the clinical condition to allow faster reconstruction and speedy healing of jawbones.
Step 5- Maintenance & Follow Up
Following the completion of implants, patients are advised to thoroughly clean around the implants as instructed by our dental hygienist. Patients will be called for the regular review so that the health and the integrity of the soft tissues and other bones can be evaluated.
If you lose one or more teeth due to sudden trauma, injury or spread of infections, as in case of periodontal diseases; dental implants can help you regain your self-confidence back. Thus, dental implants are recently designed small titanium screws, acting as anchors; to provide a strong and stable foundation, just like a natural tooth root. Thus, dental implants can help in restoring back the ability to eat everything and smile with confidence.
Technically, the jawbones around the area of missing tooth are anchored with the help of titanium screw to help create a strong foundation for artificially grafted tooth. The small posts that generally protrude through the gums are then attached to the new artificial tooth, which will help to preserve facial counters, prevent further bone loss and restore functional attributes of missing tooth.
What are dental implants for?
Dental implants can be used for:
Single tooth replacement
Multiple teeth replacement
Full mouth reconstruction
Do dental implants look real?
Implants are like your own natural teeth. In fact, you wonā€™t notice the difference probably. Their functional attributes are also same as natural and hence you can virtually eat anything, as you were eating, before you lost your teeth.
How is the dental implant procedure being accomplished?
A procedure of dental implants will typically be accomplished in two phases and the process may require up to seven months for the entire process to be complete; depending upon the rate of healing. During the procedure, local anesthesia will be offered for numbness. An incision will be made through gum around the site of tooth loss, to allow implant to be screwed on place. An enriched fraction of dental pulp derived stem cells, along with your own platelet rich plasma will be introduced at the targeted site to allow speedy integration of implants through generation of new bone.
In the second phase of implants; a small incision is being made around implants, with the application of local anesthesia. A small extension will be placed on the implants; this is where the crown will be placed.
How long do dental implant take?
Since every individual is unique, the procedure that is being offered will also be very specific. There are several factors, which determine the length and the treatment outcome. They may include:
Overall Dental Health.
Number of teeth and type of teeth that are being replaced.
Whether teeth need to be extracted and/or bone grafting needs to be done before procedure, etc.
Is the treatment painful?
As with any other dental procedure, there can be slight discomfort; the extent and severity of which, may vary from patient to patient. However, latest technologies that have implanted herewith are committed to make dental procedures, as pain free as possible. With reference to many of the reported incidences, slight swelling, bleeding can be experienced post treatment; however, it is reported to get subsided within few hours post treatment.
How stem cells therapy can be useful in undergoing Craniofacial Tissue Regeneration?
Stem cells are known to be the most potent cells in our body with the ability to retain their number back, through mitotic cell division and can undergo directed differentiation into variety of other specialized cells. Mesenchymal stem cells isolated from dental pulp tissue are found to be very helpful in guided tissue regeneration in the craniofacial cavity. However, it has as well been very apparent in different bench side studies that stem cells behavior and differentiation ability can be enhanced significantly through generation of suitable three-dimensional microenvironment. This microenvironment can be constructed through biomimetic scaffolds made up of All/Xeno/Auto substitutes in order to enhance and speed up the process of tissue reconstruction.
Is there any special care I need to take with my implants?
Dental implants should be cared just like your natural teeth. In general, it is advised to brush twice a day, flossing regularly to prevent further plaque formation and schedule routine dental examinations.
How much will the treatment cost?
Since, it is very difficult to accurately determine different treatment costs without clinically examining the patient; we would advise patients to undergo detailed consultation; wherein, we would assess the issue, through in person consultation, in order to evaluate the cost of treatment.