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Exosome Therapy

With the advancement of technologies and expertise over the period, Advancells mesenchymal stem cell-derived Exosome Therapy offers a targeted, error-free delivery mechanism for a variety of payloads including but not limited to siRNA, mRNA, proteins, small molecules, and genes.
With our advanced technology, we can efficiently isolate high-quality exosomes from several different stem cell types as per applications, along with multiple biological fluid samples like plasma, urine, serum samples, cerebrospinal fluid (CSF), ascites, saliva, and plants.

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Summary Of Exosomes For Therapy Or Delivery Vehicle

  • Exosomes can be delivered to carry a variety of therapeutic agents for delivery to specific tissues within the body.
  • Stem cells derived exosomes have been proven less immunogenic than cells. Thus, “off-the-shelf” products may readily be developed using exosomes with negligible immunogenicity.
  • With the stability data performed at our end, these exosomes can be actively stored at -20oc for up to 6 months without the use of any cryo-protectant; and without any active loss of their biochemical activities.
  • One of the important features of exosomes is their ability to encapsulate and further protect their active ingredients from degradation.
  • With mass scale expansion of mesenchymal stem cells, it is possible to isolate large quantities of exosomes from the secretome.
  • By using exosomes isolated from different producer stem cells, the region of their activity can be altered and/or specified.

How Do We Isolate Exosomes?

Out of many methods standardized for the isolation of exosomes, size-based exclusion of qualified exosomes is found to be the most efficient, safe method for therapeutic applications. We use an advanced ultrafiltration technique to isolate exosomes based on their molecular weight, by choosing suitable membrane filters. The process does not require any special equipment and is less time-consuming, and more cost-effective as compared to other existing methods of isolation of exosomes like ultracentrifugation, tangential flow filtration, etc. The below-mentioned pictorial presentation will help you understand, how easily exosomes are separated from mesenchymal stem cells.

How Exosomes Are Characterized At Advancells

It has been proven that the characterization of exosomes isolated from mesenchymal stem cells sourced appropriately is important for early detection, disease monitoring, and the development of effective therapeutics. To better define exosome characterization and facilitate the downstream application, Advancells has formulated a variety of protocols to better define the characteristics of exosomes based on various technologies.

The technologies that we have applied for exosome characterization shall include nanoparticle tracking analysis, FACS sorting, western blotting, and MASS spectrometry (MS) analysis.

One of the well popular methods of counting nanoparticles based on highly sensitive visualization is Nanoparticle Tracking Analysis. The method is based on the principle of direct detection of the light beam that is scattered through an exosome or microvesicles in liquid suspension. The scattered light is collected and detected with the help of a light microscope and then imaged.

A graph mentioning plots of scattered light spots and their speed of motion provides the data that facilitates the determination of total practice count and size distribution.

Exosome Drug Delivery Technology Platform

Exosomes are nanosized information packages released by stem cells and other cells while communicating with each other. These packages are released in extracellular spaces during cellular communication and are absorbed by other cells in a functionally relevant manner. These extracellular vesicles are preloaded with a variety of information including proteins, genetic material, etc. with the ability to hijack other cells and induce them functionally to behave the way is required. Thus, under certain conditions, exosomes generated from stem cells can be manipulated in different ways to carry different cargoes. With this principle, a wide variety of therapeutic molecules, including stem cells can be loaded into exosomes to protect the active molecules from breakdown and to target a specific area of the body. Advancells has therefore developed a technology to produce various active therapeutic components from cultured and the most potent mesenchymal stem cells, at a commercially relevant scale.

The ability to produce commercially valuable therapeutic products from stem cell-derived exosomes demands highly potent stem cells that are appropriate sources, isolated as per regulatory norms, manufactured in GMP grade facility, grown in the serum-free conditioned medium; and already having demonstrated patient safety. The mesenchymal stem cells, isolated and cultured at our end uniquely meet all those conditions.

Specific Features

Advanced technology platform

Accurate experimental data

Highly experienced team of professionals

Whole process technical trace services

  • Exosomes loaded with stem cells are helpful in targeted delivery
  • Exosomes exhibit a strong anti-inflammatory nature due to the presence of a small molecular compound namely curcumin that is known to protect against brain inflammation evident in various neurodevelopmental and neurodegenerative conditions
  • Studies have also proved that due to the presence of active therapeutic compounds like curcumin, exosomes express a higher degree of solubility, prolonged circulation time in the body, retained activity of drug therapy, and improved brain delivery.

Exosome Surface Marker-based Characterization Through Flow Analysis

With the help of flow analysis, it is possible to characterize exosomes isolated for the very specific surface proteins; and can provide critical information related to the physiological state of the parental cell. For that matter, it is very essential to characterize isolated exosomes for surface markers to give more reliable and reproducible results.

  • Flow analysis

It is the most common and reliable method to analyze exosomal surface proteins to confirm different chemical and physical characteristics of exosomes in suspension. The principle of this method is based on the light scattering properties of vesicles to analyze different exosomes based on size, once they are tagged with specific fluorescently labeled antibodies. Accordingly, we used three different antibodies, namely CD81, CD9, and CD 63 to confirm their presence.

  • Western blotting

The method is widely used for detecting specific proteins in a given sample. It is based on the semiquantitative analysis of protein based on the size and intensity of color. We used the western blotting technique to identify the presence and expression level of a specific single protein on the surface of exosomes.

 
Advancells is a strong advocate of greater transparency in medical procedures in general and Stem Cells Procedures in particular. As a part of our effort of bringing transparent procedures to our clients, we provide each client a Third Party Certificate (from an internationally accredited lab) for the cell count and viability of the cells that we are able to process from the autologous cell source of the patient. To view the sample copy of the certificate  click here

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