How To Choose Right Cells?
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Choose the right cells in the right format
If you are a part of an industry that is dealing with the purchase of fresh and/or cryostored cells; then choosing the right ones will not be as simple as one may think! There may be a number of factors that should be taken into account, before your final decision; such as cell type, functional aspects, final application, logistic as well as flexibility during transportation, etc.
Accordingly, we have come up with some important distinguishing criteria that are important to understand before choosing cells.
|1. Can be stored at -196 degree centigrade in liquid nitrogen for years.||1. Need to be consumed within 24 hours, when suspended in normal saline; in a ready to inject form.|
|2. Cryostored cells need to be revived back to their metabolic state; to be able to use for therapeutic purpose.||2. Fresh cells, being in their growing metabolic stage of cell cycle, need no further manipulation; before being used for therapeutic applications.|
|3. Due to excessive freezing and thawing, number of dead cells gets increased in the suspension; leading to cell debris. This debris, if not treated properly can lead to immediate anaphylactic reactions, post injections; like severe chills, fever, allergy etc.||3. Cellular viability, in terms of number of live cells is being controlled in suspension of fresh cells; and hence, are safer to inject.|
|4. The cryopreservation procedure, demands for the use of a cryoprotectant, DMSO (Dimethyl Sulfoxide). Studies have confirmed that DMSO can be neurotoxic, and needs to be washed properly; before preparing a cell suspension. DMSO induced toxicity can cause adverse effects like fever, chills, death in extreme conditions. It has further been suggested that cells have to be processed under expert scientific guidance, in GMP grade laboratory.||4. No chemicals need to be added in suspension of fresh cells and hence, no risk of any toxicity effects. Moreover, cells being in their highest metabolic state can reach at the targeted site at a faster rate. The functional efficiency is found to be higher in fresh cells than their cryopreserved version.|
|5. Regulatory guidelines strictly specify restricted use of cryopreserved cells for local and/or targeted applications, such as through intra-thecal or intra-arterial route.||5. There are no such restrictions in fresh cells.|
|6. Longer the cells are locked in their stage 0; more time they require to back to their metabolic state, with higher number of cellular deaths during the transition.||6. No cell death, as all the cells are live and in active state of metabolism.|