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Stem cell therapy for Critical limb Ischemia(CLI)

Stem Cell Treatment For Critical Limb Ischemia

Critical Limb Ischemia is the condition with chronic ischemic at-rest pain that may progressively lead to severe skin ulcers, gangrene, or limb loss. The condition is typically related to the objectively proven severe blockage in the artery. The disease implies, chronicity and is usually associated with multiple sites of arterial obstruction that severely reduced blood flow to the tissues. The disease is clinically manifested as rest pain, non healing wounds and/or tissue necrosis.

Ischemic pain can be associated with the burning sensation in the ball of the foot and toes, which can be worsened at night may be because of the loss of gravity assisted flow to the foot. In the past few years the most significant change in the treatment of CLI has been the increasing tendency to shift from bypass surgery to less invasive stem cell treatment procedure as first-choice causing revascularization of the damaged tissue and removal of plaque deposits to a certain extent.


Major Manifestations of CLI, in patients include the following:

  • Pain in the legs and feet when the person is at rest and not moving (also called "ischemic rest pain")
  • Sores or infections on the legs and feet that won't heal
  • Faint or absent pulse in the leg or foot below the blockage

Conventionally only bypassing the blocked artery with the help of major surgery or limb loss were the two major consequences, the patient had to face in order to avoid further progression. However, with the recent development of stem cell technology, people are now hopeful to be benefitted with the treatment that can practically reverse the damage.

Stem cells are the mother cells of the body, mainly differentiating into the required types of cells upon channelizing through proper method. Research has evidently suggested that once inside the body, they can promote revascularization of new artery by secreting growth factors and cytokines to which facilitate angiogenesis. The treatment reassures the safety of the patient.

The stem cell therapy approach mainly focuses on delivering the sufficient number of Adult Autologous Stem Cells which will travel into multiple areas of the damaged parts. .

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Stem Cell Treatment

Stem Cell Treatment For CLI: Advancells Protocol

Stem cell treatment at Advancells involves exploitation of the most potent stem cells isolated from patients own Bone marrow or Adipose tissue or may be both depending upon the assessment. Our top priority is to provide very comprehensive, individualized treatment pattern which is best suited for the patients undergoing treatment.

Since body's own cells are used the technique is very safe with no side effects, non invasive and effective.

Step 1- Qualification For The Treatment

A safety and efficacy assessments will be prescribed by our experts in order to investigate the stage of severity and qualify the patient for the treatment. Following pretreatment assessments can be suggested depending upon the patient's medical and family background:

Pre-Treatment Assessments

  • Routine Blood Tests
  • Routine Urine Analysis
  • Infectious disease testing
  • Physical Examination
  • X Ray
  • Ankle- Brachial Index

Pre-Operative Assessments

  • MRI
  • CT-Angiography
  • Medical History
  • Counselling

As per these results, the patient will be counseled further for final decision of protocol.

Step 2- Source Extraction

With the physician's approval and guidance, the source from which stem cells are to be collected is decided. In general patient can be prescribed, stem cells isolated from Bone Marrow or Adipose Tissue, as these are the most potent autologous sources available. However, in certain cases, both sources can be utilized for a better outcome. Doctors can also suggest stem cell stimulation therapy for some, depending upon the severity and requirement.

The specified amount of samples will be extracted from the patient's own body on the intimated date and time, with the application of local anesthesia. The entire procedure takes around 7-8 hrs, however, in case of extracted from both the sources the patient has to be with us for a little longer time.

If you are curious to know more about the procedure, kindly refer our detailed snapshot below :

Stem cell therapy Procedure for Critical limb Ischemia(CLI)

Step 3- Laboratory Processing

The extracted sample, will be sent to the government approved cGMP laboratory for processing. The sample processing will be done in a state of the art class 10,000 clean room in compliance with the ISO and GMP standards.

The sample will undergo minimum manipulation in our complete automated Sepax technology to be enriched with the pure population of stem cell culture. The isolated stem cells will be characterized for quality, purity and viability.

The client will be given a third party certificate from Internationally Accredited Lab for the quality purpose.

Step 4- Stem Cell Implantation

Once stem cells are enriched and ready to be injected back into the body, we have worked on different modes of implanting them. However, depending upon the patient's physiological and mental well being, one of the following will be finalized by our experts.

Follow up Treatment

In a post treatment, care, the patient will be asked to visit the doctors after a specific period of time, for some rehabilitation programs, including behavioral and emotional development, Communication and social Interaction, Self regulation, Cognitive & Adaptive skills.



CLI Stem Cell Treatment Results

Patients who have undergone treatment with us are getting better clinical benefits such as improvement in short term memory, getting better control over the daily performance. They have observed to achieve emotional stability.

The analysis of protocol for vascular surgery by the University of Crete in Greece was recently published as "Autologous Mesenchymal cells for the treatment of patients with critical limb ischemia". The results show the possibility of a promising treatment. (Read more...)



Frequently Asked Questions

  • What Is Critical Limb Ischemia?
  • It is the most severe and progressive from of arterial occusion disorder. Patients with CLI will have severe leg pain at rest mainly due to poor blood flow. If unattended for a longer period of time, it can form severe gangrene leading to limb loss.
  • What are the risk factors associated with the disease ?
  • Critical Limb ischemia is the end stage disorder of atherosclerosis. The most commonly listed risk factors associated with the disorder can be age, smoking, overweight, diabetes, high blood pressure, high cholesterol etc.
  • What are the signs and symptoms of the disease?
  • CLI is more like to be found in patients with the history of high blood pressure, high cholesterol or arterial blockages. Out of the many prominent features the most obvious are pain or numbness in the feet, shiny, dry skin of the foot, Thickening of the toenails, Opene sores or ulcers, Dry black skin of the legs.
  • How is it conventionally treated?
  • Conventionally Angioplasty, Stents or bypassing the artery through surgery are pprescribed depending upon the severity of the disease. However recovery or reversal of damage is not guaranteed, additionally these approaches are associated with many side effects.
  • What is the final outcome of its progression ?
  • Since it is a progressive disorder, if ulcers left untreated for a longer period of time they can lead to limb loss.
  • How can stem cells be beneficial to treat?
  • Undifferentiated adult autologous stem cells are mutable and can metamorph into countless number of cells as per the requirement. This remarkable propery of stem cells can be exploited for treating variety of diseases. These cells can be isolated from the most potent sources of our own body known as Adipose Tissue and Bone Marrow. Upon implanting back into the body, these cells can rapidly multiply with the great speed to restore damaged fibres, revasculatur to by pass the blocked artery as well can improve damaged function.
  • Why is stem cells treatment better than conventional treatment approach?
  • Conventional treatment approach highly invasive associated with many side effects. Although stem cells can naturally heal the body from damage and regenerate lost neurons to improve impaired functions. Additionally since body's own cells are used for repairing, the entire treatment is minimally invasive without any side effects.


What can you expect?

Many CLI patients after stem cell treatment reported to be exhibiting:

  • Improved vascularization
  • Improved Angiogenesis
  • Reduced pain
  • Improved wound healing
  • Improved self regulation
  • Improved quality of life
  • Complete stoppage or progression of the disease