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Diabetic Foot Ulcer


Stem Cell Treatment For Skin Burn

Burn is a kind of injury affecting our skin and other organic tissue of the body mainly due to heat, radiation, electricity and/or fiction. Additionally, injuries due to continuous exposure to UV radiations, chemicals fumes as well as respiratory damage resulting from smoke inhalation can also considered as Burn Injuries.

Burns are among the most common house hold injuries. Even globally, burns are a serious public health issue. As per the WHO report, an estimated 2, 65,000 deaths occur each year for fires alone. Additional 10% deaths were reported to be from scalds, electrical burns and other.

The kind of wounds by burns is characterized by severe skin damage due to death of the tissue cells. Since the skin is the largest organ of the body with many important functions such as protection, temperature regulation, sensation, storage of water, etc.

A severe burn can not only alter the physical structure but also the emotional well being of the person as well as his entire family. People with severe burn injuries may be left with the loss of physical abilities, loss of mobility, disfigurement, scarring, etc. Sometimes, the injury can penetrate into the deep germ layers causing muscle and tissue damage, affecting every system of the body.

Burns are classified as first, second and third degree depending upon the level of penetration and severity:

First Degree Burns: - They are also known as the superficial burns associated with minimum outer layer of the skin damage. The signs of them include redness, minor inflammation, and dry peeling skin. Since the burns are affecting only the top layer of the skin, the signs can disappear within three to six days with simple remedies.

Second Degree Burns: - These types are more serious, extending beyond the top layer of the skin. The extensive damage causes the skin to become extremely red and blistered giving burn or wet appearance. The worse the blisters are, the longer the time will be required to heal. Skin grafting from another area is advised for some of the severe cases such as faces, hands, buttocks, groin and feet.

Third Degree Burns: - These are the worst burns, causing severe damage to every layer of the skin. The damage can even progress to the bloodstream, major organs, and bones leading to the death. There can be the misconception that the damage can be severely painful. However, since nerves are affected, the burnt organ is almost pain free.

Generally, second and third degree burns need extensively specialized treatment as the age of the victim and percentage of the surface area that has burnt are the most important factor affecting the outlook of the injury. All the burns carry the risk of infection more than a cosmetic issues, this can even lead to organ loss due to sepsis or death.


Symptoms of Pressure Ulcers

    Burns are the severe skin scars causing major disfiguration with obvious issue, you can expect:

  • Blisters.
  • Pain ( Although the degree of pain is not dependent upon the severity of the burns, the most serious burns can be painless).
  • Peeling, red,patchy skin.
  • Swollen, white or charred skin.
  • Slight wheezing, difficulty in breathing and coughing.
  • Dark, carbon stained mucus.
  • Shock as in pale, clammy skin, weakness.

With the advancing technology, the treatment implementing Platelet Rich Plasma, is gaining popularity as a biological booster to accelerate body's delayed healing mechanism and maximise cell proliferation. The use of PRP is a very efficient technique being employed since 1990's to promote healing of the soft tissues as well as wounds and is categorically attracting many doctors, due to it's incredible outcomes; that have been observed through various clinical trials The PRP is a platelet concentrate with liquid plasma portion containing various essential growth factors, important to promote cell recruitment, multiplication as well as specialization, for faster healing. The PRP injections are prepared from the patient's own peripheral blood under strict asceptic conditions, following good laboratory practices. It is one of the best point of care technologies, exploiting patient's own activated platelets; that when infused back into the patient, can release various growth factors at the site of injury. This can in turn stimulate the microenvironment, cytokines and resident stem cells to boost initial healing processes such as inflammation, repair and remodelling. Various studies have indicated that the PRP shots have augmented healing, improved function by promoting the proliferation of reparative cells and reduce the pain associated with various maladies including but not limited to orthopaedic, wound healing, diabetic foot and sports injuries. So far so good, no adverse effects have been reported nor have we came across any after the PRP treatment, as we are exploiting patient's own blood, for which they should have no reaction to!

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PRP Treatment For Skin Burn: Advancells Protocol

The treatment at Advancells is focused mainly on improving the patient’s quality of life along with repairing the damaged tissues and restoring its lost function. For the diabetic foot, wounds and other open sore; we have designed a specialized treatment that employes patient's own PRP. Which is actually a plasma rich in the platelets. These pletelets can secrete growth factors and cytokines enabling faster healing and regeneration of damaged cells.Various studies have confirmed that the platelets play a pivotal role in the process of wound healing by initiating the inflammation. Our top priority is thus to provide very comprehensive, individualized treatment pattern which is best suited for the patients undergoing treatment, aiming their maximum recovery; according to the pathophysiology of the injury.

At Advancells, in choosing the treatment strategy, we give special consideration to the stage of wound and accordingly the purpose of the treatment such as protection, moisturization, removal of necrotic tissue etc. An algorithm for assessment and treatment is made accordingly. As a part of our treatment, patient's peripheral blood is withdrawn to prepare autologous plasma concentrate highly rich in platelets possessing various growth factors. In general for the treatment of DFU, PRP is infused locally around the wound to speed up the process of effective healing.

Advantages of PRP Therapy

  • Simple, non surgical procedure
  • Safe and reliable results
  • Very quick recovery period

Step 1- Qualification For The Treatment

A safety and efficacy assessments will be prescribed by our experts in order to investigate the stage of severity and qualify the patient for the treatment. Following pretreatment assessments can be suggested depending upon the patient's medical and family background:

Pre-Treatment Assessments Wounds

  • Infectious Disease Testing
  • Routine Blood tests
  • Physical Examination
  • Urine analysis
  • X ray
  • MRI
  • CT-Scan
  • Non Invasive Vascular Studies
  • X ray
  • Medical Reports & History

As per these results, the patient will be counseled further for final decision of protocol.

Step 2- Source Extraction

PRP is rich platelet concentrate, with the seven essential protein growth factors which can rapidly initiate cascade of reactions for wound healing; additionally it includes three very fundamental proteins that can act as a "Cell Adhesion Molecules" to activate signal transmission. This activation can express the gene, directing the cellular proliferation, matrix formation, osteoid formation and collagen synthesis; which promote tissue repair and regeneration. Reasearch has as well confirmed that after injection in the body, PRP can actively secrete the growth factors within 10 mins while more than 95% of them are secreted within 1 hr after activation.

If you are curious to know more about the procedure, kindly refer our detailed snapshot below:

stem cell treatment for ALS

Step 3- Laboratory Processing

PRP is generally obtained from the peripheral blood of the patient drawn at the time of treatment. Approximately 30CC will be drawn from the patient depending upon the baseline platelet count of the individual.In order to prevent unnecessary activation of platelet, the venous blood of the patient will be collected using suitable anticoagulent. A specialized process will be employed to separate the platelet rich plasma from the whole blood of the patient; which is then infused around the wound or other targeted area under the guidance of specialist to promote faster wound healing.

The sample will undergo minimum manipulation to be enriched with the pure population of platelet; which would the best possible treatment for faster wound healing.

The client will be given a third party certificate from Internationally Accredited Lab for the quality purpose.

Step 4 - Implantation

Once the PRP ready to be injected back into the body, we generally put them back through local intradermal administration through syringes around the wound.

  • Intra-Dermal Administration:- The technique is used in case of open sores, wherein PRP will be infused locally in and around the wound area.

Follow up Treatment

Post treatment, the patient will be asked to visit the doctors for reclamation therapies such as Physiotherapy, patients counselling etc. for accelerated recovery.

* Patient age, size of burn area and burn depth is the most important factors that influence mortality and morbidity after thermal injuries.


Gastric Ulcers Treatment Results

Patients, who have undergone a PRP treatment with Advancells for their wounds such as diabetic foot have acquired a very fast recovery. All of them were suffering from non healing diabetic foot during their late 50's as a major complication of Diabetes. Since the management of the wound in diabetic patients is very poor and painful, a kind of advanced technology was suggested for them as a treatment to manage the problem; which can easily take over to traditional options such as surgeries, debridement, antibacterial actions etc. A PRP was isolated from the peripheral blood of all the patients and injected around the wound through microneedles. The results showed that 100% ulcers completely closed after about 7-8 weeks. Although, patients response to the wounds was not typical; no adverse effect has been observed till now. The method is completely authentic, approved by the regulatory authority as a novel and minimally invasive method.

Patient's Stories

Mr. Ramesh Sharma (name changed), 60 years, Diabatic foot

" In 2006, when Mr. Ramesh Sharma had been diagnosed with the diabetes, his family were shocked with the news! He was a fit, healthy man with happy family and work life. Mr. Ramesh began with the treatment that was progressing effectively. However the treatment left him with the severe diabetic foot wound on his right leg. The wound was causing him severe pain disturbing his routine life. He had previously sought treatment at different centers before finally being referred to Advancells........"


Frequently Asked Questions

  • Why PRP Gel for Burn wounds?
  • 1. Use of new cutting aged technology in activation of PRP and gel preparation
  • 2. PRP gel is easy to apply over wound
  • 3. Very quick method of treatment (time varies as per wound area)
  • 4. Less or no invasive procedure of wound dressing using PRP gel
  • 5. Improves burn wound-healing time after the application of PRP
  • How PRP Gel works in burn injury?
  • Platelet-rich plasma contains a high level of platelets, clotting factors, cytokines, and growth factors. They start releasing these activated factors 10 minutes after clotting, and more than 95% of growth factors are released in the first hour. Platelet rich plasma is stable for approximately 8 hours after preparation [1,2]
  • 1. TGF-b and PDGF in PRP are triggers cell growth and differentiation leading to early wound healing.
  • 2. bFGF, PDGF, IGF, and EGF in PRP increases epidermal regeneration
  • 3. Collagen synthesis and maturation increases due to PRP applications leading to increase collagen deposition that helps in speedy recovery.
  • 4. Enhances wound healing, promoting epithelization and angiogenesis.
  • Does multiple doses of PRP helps?
  • It has been shown that multiple dosage of PRP accelerated formation of extracellular matrix during the first half of wound healing.
  • Use of PRP over burn injury: is it safe?
  • 1. PRP is generated from patients own blood hence immunologically neutral with no cross/hyper reactivity as are easily acceptable by patients body.
  • 2. Blood Collection, PRP separation and activation including PRP gel preparation is carried out in complete sterile conditions in GLP compliance laboratory. Hence PRP gel application does not lead to infection.
  • 3. PRP may be injected intralesionally or perilesionally or in the form of a platelet gel and used topically.
  • 4. Intradermal injections of PRP at the wound site may cause inflammation with or without nerve trauma in rare cases.
  • Reference:
  • 1. Pallua N, Wolter T, Markowicz M: Platelet-rich plasma in burns. Burns, 2010; 36(1): 4–8 4.
  • 2. Yol S, Tekin A, Yilmaz H et al: Effects of platelet rich plasma on colonic anastomosis. J Surg Res, 2008; 146(2): 190–94
  • 3. Kazakos K, Lyras DN, Verettas D et al: The use of autologous PRP gel as an aid in the management of acute trauma wounds. Injury, 2009; 40(8): 801–5
  • 4. Loot MA, Kenter SB, Au FL et al: Fibroblasts derived from chronic diabetic ulcers differ in their response to stimulation with EGF, IGF-I, bFGF and PDGF-AB compared to controls. Eur J Cell Biol, 2002; 81(3): 153–60