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Stem Cell Therapy for Wound Healing
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Introduction

Stem cell treatment for Skin Injuries/Wounds

Skin is the largest yet delicate organ of our body, comprising of mainly three distinguished layers, namely, outer epidermis, middle dermis and the innermost hypodermis; each contributing uniquely towards maintaining structural and functional integrity of the organ. Skin has some different functional attributes, it serves as a protective covering, saving internal vital organs from physical, chemical and environmental assaults. Presence of nerve endings in the skin provides sensation, while melanin imparts color to the skin.

It serves as one of the self-renewing organs, with a strong potential to repair and heal small cuts, scratches on its own; due to the presence of resident adult stem cells. However, their number is not sufficient enough to take control over major injuries like deep cuts or abrasions, skin burns and ulcers. These skin injuries/wounds can cause severe damage to the deepest layer of the skin; thus, may compromise normal function of the skin.

Skin injuries/wounds, if ignored for a longer period of time can cause severe infection, leading to the life-threatening complications, such as gangrene, sepsis, cellulites, etc.

Repair of skin injuries can be possible in three steps, i.e. inflammation, proliferation and remodeling; and various skin cells are involved in the process of healing, such as keratinocytes, fibroblasts, vascular endothelial cells, etc. But in cases, of deeper injuries or wounds the skin repair requires, immediate medical assistance.

Some of the common categories of injuries include:

Conventionally, the only available treatment option for the above-mentioned skin issues was skin grafts, obtained from allogenic as well as autologous sources. However, the applications of conventionally available options have often been found to be associated with number of limitations, such as shortage of autografts, especially for patients with large surface area of burns, skin infections in case of allografts, aesthetically unappealing finishing, etc.

For that matter, majority of scientific community has been forced to think about the new effectively simple approach towards wound healing. Accordingly, a combinational approach of adipose tissue derived stem cells activated with his own platelet rich plasma has been proven to be effective as a potential wound healing strategy. Number of studies have been performed to support the scientific advancements and have confirmed augmented healing rates and improved functional attributes by promoting faster proliferation of lost or degenerated cells.

So far so good, no adverse effects have been encountered in any of the ongoing clinical study; nor have we come across any during our treatments!

Treatment

Stem cell treatment for Skin Injuries/Wounds

The treatment at Advancells is focused mainly on improving the patient"s quality of life along with repairing the damaged tissues and restoring its lost function. For the diabetic foot, wounds and other open sore; we have designed a specialized treatment that employes patient's own PRP. Which is actually a plasma rich in the platelets. These pletelets can secrete growth factors and cytokines enabling faster healing and regeneration of damaged cells.Various studies have confirmed that the platelets play a pivotal role in the process of wound healing by initiating the inflammation. Our top priority is thus to provide very comprehensive, individualized treatment pattern which is best suited for the patients undergoing treatment, aiming their maximum recovery; according to the pathophysiology of the injury.

At Advancells, in choosing the treatment strategy, we give special consideration to the stage of wound and accordingly the purpose of the treatment such as protection, moisturization, removal of necrotic tissue etc. An algorithm for assessment and treatment is made accordingly. As a part of our treatment, patient's peripheral blood is withdrawn to prepare autologous plasma concentrate highly rich in platelets possessing various growth factors. In general for the treatment of DFU, PRP is infused locally around the wound to speed up the process of effective healing.

Advantages of PRP Therapy

  • Simple, non surgical procedure
  • Safe and reliable results
  • Very quick recovery period

Step 1- Qualification For The Treatment

A safety and efficacy assessments will be prescribed by our experts in order to investigate the stage of severity and qualify the patient for the treatment. Following pretreatment assessments can be suggested depending upon the patient's medical and family background:

Pre-Treatment Assessments Wounds

  • Infectious Disease Testing
  • Routine Blood tests
  • Physical Examination
  • Urine analysis
  • X ray
  • MRI
  • CT-Scan
  • Non Invasive Vascular Studies
  • X ray
  • Medical Reports & History

As per these results, the patient will be counseled further for final decision of protocol.

Step 2- Source Extraction

PRP is rich platelet concentrate, with the seven essential protein growth factors which can rapidly initiate cascade of reactions for wound healing; additionally it includes three very fundamental proteins that can act as a "Cell Adhesion Molecules" to activate signal transmission. This activation can express the gene, directing the cellular proliferation, matrix formation, osteoid formation and collagen synthesis; which promote tissue repair and regeneration. Reasearch has as well confirmed that after injection in the body, PRP can actively secrete the growth factors within 10 mins while more than 95% of them are secreted within 1 hr after activation

If you are curious to know more about the procedure, kindly refer our detailed snapshot below:

Stem Cell Therapy Procedure for Wound Healing

Step 3- Laboratory Processing

PRP is generally obtained from the peripheral blood of the patient drawn at the time of treatment. Approximately 30CC will be drawn from the patient depending upon the baseline platelet count of the individual.In order to prevent unnecessary activation of platelet, the venous blood of the patient will be collected using suitable anticoagulent. A specialized process will be employed to separate the platelet rich plasma from the whole blood of the patient; which is then infused around the wound or other targeted area under the guidance of specialist to promote faster wound healing.

The sample will undergo minimum manipulation to be enriched with the pure population of platelet; which would the best possible treatment for faster wound healing.

The client will be given a third party certificate from Internationally Accredited Lab for the quality purpose.

Step 4-Implantation

Once the PRP ready to be injected back into the body; in case of open sore, we put them back through local intra-dermal administration via microneedles around the wound.

  • Intra-Dermal Administration:- The technique is used in case of open sores, wherein PRP will be infused locally in and around the wound area.

Follow up Treatment

Post treatment, the patient will be asked to visit the doctors for reclamation therapies such as Physiotherapy, patients counselling etc. for accelerated recovery.

 

Result

Skin Injuries Treatment Results

Patients, who have undergone a PRP treatment with Advancells for their wounds such as diabetic foot have acquired a very fast recovery. All of them were suffering from non healing diabetic foot during their late 50's as a major complication of Diabetes. Since the management of the wound in diabetic patients is very poor and painful, a kind of advanced technology was suggested for them as a treatment to manage the problem; which can easily take over to traditional options such as surgeries, debridement, antibacterial actions etc. A PRP was isolated from the peripheral blood of all the patients and injected around the wound through microneedles. The results showed that 100% ulcers completely closed after about 7-8 weeks. Although, patients response to the wounds was not typical; no adverse effect has been observed till now. The method is completely authentic, approved by the regulatory authority as a novel and minimally invasive method.

Patient's Stories

Mr. Ramesh Sharma (name changed), 60 years, Diabetic foot

" In 2006, when Mr. Ramesh Sharma had been diagnosed with the diabetes, his family were shocked with the news! He was a fit, healthy man with happy family and work life. Mr. Ramesh began with the treatment that was progressing effectively. However the treatment left him with the severe diabetic foot wound on his right leg. The wound was causing him severe pain disturbing his routine life. He had previously sought treatment at different centers before finally being referred to Advancells........"

 

FAQ's

Frequently Asked Questions

  • What is PRP?
  • PRP is the platelet Rich Plasma. Plasma is the liquid component of the blood, which serves as the host for all the cellular components of the blood like red blood cells, white blood cells and platelets. Thus platelets naturally make up about 10% of the blood"s cellular component. With the help of a technology, it is possible to flip the ratio of platelets upto 90% concentration. Therefore it is named as the Platelet Rich Plasma.
  • What is PRP treatment and how does it work?
  • Platelets are known as the first respondents after the injury. Immediately after the injury, the human body starts eliciting an immune response allowing platelets and other immune components to migrate at the site of injury. At the targeted site, these platelets are known to secrete various growth factors and clotting factors to initiate the healing process, promote blood vessels formations as well as collagen synthesis. These platelets are also known to trigger the secretion of many bioactive molecules, which can attract many macrophages, mesenchymal stem cells and osteoblasts. PRP is a non surgical healing treatment used in the field of regenerative medicines to stimulate and enhance healing. In a simple language, PRP is considered as the mega doses prepared from the ingredients of our own body for our own healing.
  • What types of conditions are treated with PRP?
  • Based on the current research, all the orthopaedic related problems such as soft tissue injuries, tendonitis, ligament sprains and muscle tears can be treated well with PRP treatment. It is as well found to be very useful in anti aging treatment, hair restoration and wound healing such as diabetes foot.
  • Is PRP treatment painful?
  • Patient typically tolerates the treatment well both with and without anaesthesia. However post injection swelling in expected given the PRP induced inflammatory response. But these discomforts such as swelling are observed in the typical injected area which will not be for more than 48 hrs.
  • How long does it take the PRP to work?
  • Many of the people have notices some or the other kind of improvement depending upon the problem by 2-3 weeks after the PRP treatment. Symptom improvement has been observed to be slow and subtle with the passage of time, with usual complaint of original pain being replaced by the soreness. Increased endurance and strength are typical observation immediately after the treatment.
  • Preparation of PRP?
  • A small amount of blood is withdrawn generally 30 ml from the peripheral vein of the patient. The blood is then placed in the centrifuge, to separate platelet from rest of the components. On the therapeutic aspects, platelet count is enriched 5 fold with 10-15 fold decrease in the volume after the processing. The entire process takes not more than 20 mins to increase the concentration of platelet along with the inherent stimulation of stem cells for quick proliferation, differentiation and regeneration. The concentrated PRP is then injected around the area or in the joints to speed up the process of healing.
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What can you expect?

Many patients after stem cell treatment reported to be exhibiting:

  • Inflammatory process activity decreases
  • Morning stiffness, joint tenderness, oedema is diminished
  • Patient's functional capacity has been improved tremendously
  • Considerable redevelopment of cartilaginous tissue observed from X-Ray analysis that can avoid further damage to the joints.
  • In most of the cases, complete discontinuation or considerable reduction of anti inflammatory drugs has been achieved.
  • Quality of life has been improved in patients achieving 100% target.
  • However, a complete restoration of the central nervous system, is the prolonged process and requires lots of patience and repetition of treatment if required or suggested.