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STEM CELL PROCEDURE

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How and where is my therapy done?

Making a decision to undergo stem cell therapy is a huge step. The Advancells truly admires your spirit of being healthier you!

Unfortunately, it is highly unlikely that you will wake up one morning, cured of all your problems! And hence,  we will help you identify some telltale signs further indicating you that you are ready to move on with the stem cell therapy.

Once you are ready to be treated under the expert guidance of Advancells-stem cell specialists; you will be counselled further through our expert specialist, who will detail you about the procedure, how the procedure is being done, what can you expect after treatment, what are the possible side effects of the procedure and which type of cells are the best suited to treat you well. Post counselling session, you may undergo some radiology and/or pathological investigations. Depending upon the clinical assessments and evaluation of medical history; you will further be intimated about your scheduled date and time of therapy. 

From the moment of your enrollment till the time of your exit from hospital set up; you will always be assisted with our representative to make sure that all your queries, doubts and worries will get resolved, and make you feel more confident and at peace. 

 

Stem Cells Procedure: Advancells Protocol

The entire medical procedure at Advancells consists of 4 steps:

Source extraction

Isolation, analysis and concentration of the stem cells in the laboratory

Stem cell implantation

Post treatment care

Step 1- Qualification For The Therapy

Once you are counselled by our expert paneled doctor, and are convinced to go ahead with stem cell therapy; you will be prescribed some safety and efficacy assessments, which will further help us to know more about the severity of your disease. Further to which, some of the basic tests can also be prescribed that can help us to investigate, whether you are eligible for the treatment. 

As per the regulatory guidelines proposed by CDESCO and GMP, you will be informed about the consequences, in the context of possible risks and benefits. Although, stem cell therapy has been referred to be as completely safe and risk-free procedure; under the moral and legal premise of informed consent, you have the right to make decisions about your own health and medical condition. 

Pre-Treatment Assessments

  • Routine Blood Tests
  • Infectious Diseases Testing
  • Urine Analysis
  • Physical Examination
  • X Ray

Pre-Operative Assessments

  • Surgical Correction
  • MRI
  • CT Scan
  • Medical History

Step 2- Source Extraction

As per our standardized protocol, discussed herewith specified amount of sample will be extracted from patient’s own body with the application of local anesthesia. The entire procedure should not take more than 7-8 hrs; however, in case of certain critical cases, patient may have to wait for a little longer period of time. 

Bone Marrow Derived Adult Stem Cells Extraction

One of our senior Neurosurgeon/Orthopaedic surgeons will extract the bone marrow from the iliac crest (Hip Joint). Normally, the procedure takes around 30-45 minutes. Local anaesthesia is first administered on the area of the skin that is to be punctured. A bone marrow extraction needle is then used to extract about 100-120 ml of bone marrow. Option of general anaesthesia is available for patients who need it. Once the extraction is done, the sample will be immediately transferred to our clean room facility for processing.
More detailed information on the bone marrow collection procedure is available in the Bone Marrow Informed Consent document (PDF file).

bonemarrow stem cell extraction

Isolation of stem cells from Bone Marrow
In a clean room, stem cells will be separated from other unwanted mature blood cells and plasma. The processing of samples will be done as per cGMP standards under sterile conditions. Utmost care will be taken to make sure that stem cells do not get contaminated by any kind of infections. Post processing all samples will be tested for quality and quantity of stem cells.

Adipose Tissue Derived Adult Stem Cells Extraction

One of our operating surgeon/cosmetic surgeons will extract an adipose tissue from an area of the body, which is generally pre-umbilical area; where sufficient amount of fat is available. Once the location is confirmed, a patient will be asked to lie in a supine position. He will be given a local anaesthesia. A 60 CC cannula is inserted under subcutaneous layer and approximately 100 CC adipose tissue is collected. Once the aspiration is complete, the sample will be immediately transferred to the clean room for further processing.

More detailed information on the adipose Tissue collection procedure is available in the Adipose Tissue Informed Consent document (PDF file)

adipose stem cell extraction process

Isolation of Stem Cells From Fat
Fat is abundant source of progenitor/stem cells; which will be separated from other mature cells such as macrophages, adipocytes etc. in our clean room facility. The processing of sample is done as per approved cGMP standards under sterile conditions. Utmost care is taken to make sure that stem cells do not get contaminated by any kind of infections. Post processing, all samples will be tested for quantity as well as quality.

Step 3- Laboratory Processing

Once, the sample is being extracted from the patient; it will be sent to our GMP compliant processing unit; and will further be processed for stem cell enrichment. We ensure each step is being quality controlled, recorded and pass by our authorized QA; this has been helpful to maintain completely safety and quality of stem cells that are being dispatched from our end.  Our procedures are completely automated and are being performed in completely closed system, to avoid structural as well as functional manipulation of stem cells. Right from the sample receipt till the time of stem cell dispatch, we don’t compromise at any step. All the procedures are being performed by our experienced scientists and are documented for future analysis.

We value our clients and appreciate their belief on us; hence, the client will be given third party certificate from NABL certified lab, further assuring you complete safety. We are the only providers of cell count certificate to our clients, which details about the number of viable cells offered.

If you are curious to know more about the procedure, kindly refer the detailed file:

Step 4- Stem Cell Implantation

Once stem cells are enriched and ready to be injected back into the body, we have worked on different modes of implanting them. However, depending upon the patient’s physiological and mental well being, one of the following will be finalized by our experts.

 

Stem Cell Implantation

Stem cells can be implanted in following major ways:

1. Intravitreal Infusion

The intravitreal drug delivery has become the most reformed technology in treating some of the ophthalmic conditions such as macular oedema, retinal vein occlusions, etc. These are the conditions wherein single dose administered through intravitreal region is more effective than repeated injections through retrobulbar mode.

The inside of the human eye is filled with a jelly like fluid known as “Vitreous”; an intravitreal injection is the direct shot of stem cells into the vitreous structure near the retina at the back of the eye. The entire procedure takes no more than 15-20 minutes.

  • The procedure requires dilation of the pupil, which can be acquired by placing specific eye drops in the eyes.
  • Once pupils are dilated, the patient will be allowed to lie in a comfortable position and anaesthetic drops will be placed at the interior surface of the eye.
  • After cleaning both the eyes and the eyelids through the iodine antiseptic solution, with the help of a small device the eye and the eyelids are kept open during the entire procedure.
  • A shot of stem cells will be injected directly into the vitreous region with the help of small needle; a patient may feel slight pressure on the eye without any pain.
  • The eyes will be checked properly, some antibiotic ointment can be placed in the eyes if required.
2. Retrobular Infusion of cells

The retrobulbar injection is a very common method routinely exercised by many ophthalmologists for the infusion of stem cells to treat diseased eye such as Macular Degeneration, Glaucoma and other eye disorders.

In this particular procedure with the local anaesthesia needle will be inserted inferior to the maximum diameter of the globe and perpendicular to your face. Once past the axis; the needle will be angled medially and superiorly. Final needle position will be within the muscle cone. Concentrated number of stem cells will be injected in the area near macula under the eye.

This method of administration is preferred because of the higher number of cells directly into the targeted area to maximise the output. Advancells can also give a combination of two protocols depending what the doctor feels is right for the patient.

Follow up Treatment

In a post treatment care, the patient will be asked to visit the doctors after a specific period of time, for some rehabilitation programmes including Behavioural and emotional development, Communication and social Interaction, Self regulation, Cognitive and Adaptive skills.

Treatment Disclaimer

Before undergoing this process; a patient must understand that stem cell therapy is an experimental therapy that may fail to work at certain times.

A patient might be refused treatment or the proposed protocol might change depending on the patient’s health condition at the time of procedure.

At Advancells, our primary motive is to offer the best technology available; safety of which has been proven across the world.

 

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Advancells

A-102, Sector-V Noida-201301, UP, India

Tel: +91-9654321400

Email: info@advancells.com 

 

 

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"Advancells is a strong advocate of greater transparency in medical procedures in general and Stem Cells Procedures in particular. As a part of our effort of bringing transparent procedures to our clients, we provide each client a Third Party Certificate (from an internationally accredited lab) for the cell count and viability of the cells that we are able to process from the autologous cell source of the patient. To view the sample copy of the certificate  "click here"

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